Journal: bioRxiv
Article Title: Lipid lowering alone fails to limit atherosclerosis progression and neutrophilic inflammation in middle-aged mice
doi: 10.1101/2025.11.04.686598
Figure Lengend Snippet: (A) Brains were isolated, sectioned, and immunofluorescence was performed for lectin (green) as a marker of the endothelium and Ly6G (red) as a marker of PMN. (B) The percent colocalization of lectin and Ly6G was quantified in the hippocampus using ImageJ. (C) The hippocampus was isolated from a subset of randomly selected mice and flash-frozen for LC-MS/MS processing and analysis. Results are mean ± SEM and each dot represents an individual mouse. *p<0.05, ****p<0.0001 Student’s t-test.
Article Snippet: After 24 hours, sections were incubated with a secondary antibody solution containing Alexa Fluor 594 anti-rat antibody (cat# A-11007, Thermo Fisher Scientific, Waltham, MA, USA) at 1:300 and a fluorescein-conjugated Lectin antibody (cat# FL-1171-1, Vector Laboratories, Newark, CA, USA) at 1:100 for 2 hours at 30C.Stained brain sections were visualized on a Leica Thunder Imager using LAS X software.
Techniques: Isolation, Immunofluorescence, Marker, Liquid Chromatography with Mass Spectroscopy